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lncR-GAS5 upregulates the splicing factor to impair endothelial autophagy, leading to atherogenesis
《医学前沿(英文)》 2023年 第17卷 第2期 页码 317-329 doi: 10.1007/s11684-022-0931-4
关键词: lncR-GAS5 miR-193-5p splicing factor SRSF10 autophagy atherogenesis
Huiwen Ren, Can Wu, Ying Shao, Shuang Liu, Yang Zhou, Qiuyue Wang
《医学前沿(英文)》 2020年 第14卷 第5期 页码 642-650 doi: 10.1007/s11684-019-0719-3
关键词: type 2 diabetes mellitus diabetic kidney disease miR-154-5p urinary albumin to creatinine ratio
《医学前沿(英文)》 页码 924-938 doi: 10.1007/s11684-023-1004-z
关键词: ovarian cancer lncRNA LOC646029 metastasis microRNA 627-3p SPRED1
RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway
《医学前沿(英文)》 2023年 第17卷 第1期 页码 143-155 doi: 10.1007/s11684-022-0929-y
null
《医学前沿(英文)》 2015年 第9卷 第3期 页码 331-343 doi: 10.1007/s11684-015-0409-8
MicroRNAs (miRNAs), an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level. miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma (HCC) and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement. miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3′- and 5′-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.
关键词: hepatocellular carcinoma microRNA metastasis cytoskeletal reorganization
MicroRNA-148b promotes proliferation of hair follicle cells by targeting
Wanbao YANG,Qinqun LI,Bo SU,Mei YU
《农业科学与工程前沿(英文)》 2016年 第3卷 第1期 页码 72-80 doi: 10.15302/J-FASE-2016089
The microRNA, miR-29c, participates in muscle development through targeting the
Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI
《农业科学与工程前沿(英文)》 2015年 第2卷 第4期 页码 311-317 doi: 10.15302/J-FASE-2015075
Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and
null
《医学前沿(英文)》 2014年 第8卷 第4期 页码 456-463 doi: 10.1007/s11684-014-0353-z
microRNAs (miRNAs) have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c (miR-200c) on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200c compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR-200c could inhibit migration and invasion by epithelial-mesenchymal transition.
关键词: miR-200c proliferation migration invasion prostate cancer Du145 cell ZEB1
hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析
徐青,付子毅,吴小莉,皇甫玉爽,凌静
《中国工程科学》 2014年 第16卷 第5期 页码 99-104
关键词: 子宫肌瘤 hsa-miR-197 生物信息学 靶基因
Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
null
《医学前沿(英文)》 2017年 第11卷 第2期 页码 214-222 doi: 10.1007/s11684-017-0518-7
MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial?mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer.
-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation
Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,
《工程(英文)》 doi: 10.1016/j.eng.2023.09.007
关键词: Traditional Chinese medicine Lycium barbarum L miR162a Osteoporosis Nuclear receptor corepressor Transgenic plants
促进肝癌致癌活性和细胞代谢的新型介质——miR-516a-3p
芮韬, 张学优, 冯时, 黄海涛, 詹少伟, 谢海洋, 周琳, 郑树森, 凌琪
《工程(英文)》 2022年 第16卷 第9期 页码 162-175 doi: 10.1016/j.eng.2021.07.020
Estimation of flexible pavement structural capacity using machine learning techniques
Nader KARBALLAEEZADEH, Hosein GHASEMZADEH TEHRANI, Danial MOHAMMADZADEH SHADMEHRI, Shahaboddin SHAMSHIRBAND
《结构与土木工程前沿(英文)》 2020年 第14卷 第5期 页码 1083-1096 doi: 10.1007/s11709-020-0654-z
关键词: transportation infrastructure flexible pavement structural number prediction Gaussian process regression M5P model tree random forest
Wenjiang LI, Fei XIE, Dongxu HUA, Chunli ZHANG, Chen DAI, Zhenyun YU, Meizhou QI, Shaojun YU
《化学科学与工程前沿(英文)》 2011年 第5卷 第3期 页码 314-317 doi: 10.1007/s11705-010-0573-y
关键词: P2O5-SiO2 hollow microspheres sol-gel/emulsion phosphoric acid
《工程管理前沿(英文)》 2022年 第9卷 第2期 页码 349-353 doi: 10.1007/s42524-022-0187-3
关键词: Brazil FPSO P67/P70 superlarge dry towing operation construction integration risk control management
标题 作者 时间 类型 操作
lncR-GAS5 upregulates the splicing factor to impair endothelial autophagy, leading to atherogenesis
期刊论文
Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes
Huiwen Ren, Can Wu, Ying Shao, Shuang Liu, Yang Zhou, Qiuyue Wang
期刊论文
Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR-627-3p/SPRED1 axis
期刊论文
MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic
null
期刊论文
MicroRNA-148b promotes proliferation of hair follicle cells by targeting
Wanbao YANG,Qinqun LI,Bo SU,Mei YU
期刊论文
The microRNA, miR-29c, participates in muscle development through targeting the
Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI
期刊论文
Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and
null
期刊论文
Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
null
期刊论文
-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation
Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,
期刊论文
Estimation of flexible pavement structural capacity using machine learning techniques
Nader KARBALLAEEZADEH, Hosein GHASEMZADEH TEHRANI, Danial MOHAMMADZADEH SHADMEHRI, Shahaboddin SHAMSHIRBAND
期刊论文
Preparation of P
Wenjiang LI, Fei XIE, Dongxu HUA, Chunli ZHANG, Chen DAI, Zhenyun YU, Meizhou QI, Shaojun YU
期刊论文